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For both cardiovascular and pulmonology applications, tubular structures such as the bronchial tree, and arteries need to be characterized. Typically, a centerline of these tubular structures is extracted before correct measurements, and associated views can be obtained (Bouix et al., 2004, Choi et al., 2002, Jiang and Gu, 2005, Schaap et al., 2009, Wan et al., 2002). An automated centerline extraction, with manual adjustment possibilities of the centerline control points is implemented in Mimics 15.0 and 16 (Materialise N.V., Belgium). This white paper describes the quantitative validation of this centerline extraction method.